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Part:BBa_K2411004:Design

Designed by: Abigail Sasdelli   Group: iGEM17_BostonU   (2017-09-27)


Forward Engineered Toehold 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is important to ensure that the toehold forms the proper hairpin loop and that the RBS contained in the hairpin loop is compatible with the system being used, in our case an E. coli derived cell free transcription translation system.


Source

This sequence was synthetically design from Green et. al. The sequence can be found in the supplementary information from the following paper:

Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.

References

Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.